130 - Comparison of multiplex RT-PCR and RNA-Seq for detection of pathogens in tracheal aspirate samples in critically ill children.

David I. Del Valle Ortiz, University of Rochester School of Medicine and Dentistry, San Juan, Puerto Rico, United States; Lauren Gunn-Sandell, University of Colorado, Aurora Medical Campus, Aurora, CO, United States; Padmini Deosthale-Bapat, University of California, San Francisco, School of Medicine, San Francisco, CA, United States; Emily Lydon, University of California, San Francisco, School of Medicine, San Francisco, MA, United States; Lilliam Ambroggio, Children's Hospital Colorado, Aurora, CO, United States; Brandie Wagner, University of Colorado School of Medicine, Aurora, CO, United States; Nadir Yehya, CHOP, PHILADELPHIA, PA, United States; Aline B. Maddux, University of Colorado School of Medicine, Aurora, CO, United States; Matthew Leroue, University of Colorado School of Medicine, Aurora, CO, United States; Eric A.F.. Simões, Children's Hospital Colorado, Aurora, CO, United States; Charles R. Langelier, UCSF, San Francisco, CA, United States; Samuel Dominguez, Children's Hospital Colorado, Denver, CO, United States; Peter M. Mourani, University of Arkansas for Medical Sciences, Little Rock, AR, United States; Christina M. Osborne, CHILDREN'S HOSPITAL OF PHILADELPHIA, Philadelphia, PA, United States